Available reagents

Licensing for the research community

Cell lines, antibodies, proteins, DNA constructs, small molecules and other research tools generated by scientists at the University of Cambridge play a key role in laboratory research. There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing, so please contact us directly with your specific needs.

Non-exclusive licences with standard terms and conditions are available for available reagents.

MOCHA Mouse Skin Fibroblast Cell Line

16 February, 2017 | Cell lines Code: CL-0385
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MOCHA is a naturally occurring mouse mutant with a null mutation in the delta subunit of AP3 complex. In MOCHA cells, the ß3 and μ3 subunits co-assemble into a heterodimer whereas the σ3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The MOCHA mouse skin fibroblast cell line can be used to investigate the assembly of AP3 complexes and AP3 function.

J. Cell Biol. 2002, 156, 327-336.

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Mouse MAC-1 monoclonal antibody YBM 15.1.6

22 December, 2016 | Code: CL-0391
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Hybridoma cell line producing YBM 15.1.6 antibodies raised against mouse MAC-1, the murine equivalent of CD11b. The YBM 15.1.6 antibodies are rat monoclonal antibodies of the IgG2b isotype.

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Mouse MAC-1 monoclonal antibody YBM 6.1.10

22 December, 2016 | Monoclonal hybridomas Code: CL-0390
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Hybridoma cell line producing YBM 6.1.10 antibodies raised against mouse MAC-1, the murine equivalent of CD11b. The YBM 6.1.10 antibodies are rat monoclonal antibodies of the IgG2a isotype.

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Tetrabutylammonium (4’methyl-[2,2’bipyridin]-5yl)methanesulfonate

15 November, 2016 | Small molecules Code: OM-0384
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Tetrabutylammonium (4’methyl-[2,2’bipyridin]-5yl)methanesulfonate is a small molecule that has been shown to act as a bifunctional ligand for iridium to control regioselectivity of iridium-catalysed borylation on cationic substrates as shown in the following publication:

J. Am. Chem. Soc. 2016, 138, 12759−12762

This purified small molecule is available for licensing. Please get in touch if you would like more information.

ligandjpg

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-5yl)methanesulfonate - Code: OM-0384']

CRISPR Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

25 May, 2016 | Plasmids Code: Undisclosed
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A CRISPR Cas9D10A-based screening approach that combines an All-in-One Cas9D10A nickase vector with fluorescence-activated cell sorting enrichment has been developed in the lab of Prof Steve Jackson at the University of Cambridge. The technique is combined with high-throughput genotypic and phenotypic clonal screening strategies to generate isogenic knockouts and knock-ins highly efficiently, which leads to minimal off-target effects. Please see the published paper describing the technology below:

Chiang, T.-W. W., le Sage, C., Larrieu, D., Demir, M. and Jackson, S. P. Sci Rep. 2016, 6, 24356.

Please get in touch if you would like more information.

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Tephrosin

25 May, 2016 | Small molecules Code: OM-0379
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Tephrosin is a plant-based rotenoid which has known anti-cancer properties. An academic group at the University of Cambridge has recently completed the novel chemical synthesis of Tephrosin. The purity of the product is in excess of 99% as confirmed by LC-MS and NMR.

Purified Tephrosin is available for licensing. Please get in touch if you would like more information.

Tephrosin ≥95% (LC/MS-ELSD)

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PC12 HD-Q23 (lines 14 & 7) and HD-Q74 (lines 10 & 1b) cell lines for Huntington’s Disease research

3 February, 2016 | Cell lines Code: CL4 and CL5 (Rub-1856-07)
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Two stable inducible rat phaeochromocytoma (PC12) cell lines expressing GFP-tagged exon 1 of the huntingtin gene with either 23 or 74 glutamine repeats (PC12 HD-Q23 or PC12 HD-Q74), driven by a doxycycline-dependent Tet-On promoter – these cell lines can be used for in vitro studies to evaluate compounds to treat Huntington’s Disease.

Download more information here.

Publications:

Wyttenbach et al. (2001) Hum Mol Genet. 10,1829-45.

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Leukocyte Ig-Like Receptor (LILR) Mouse Model

1 February, 2016 | Other Code: OM-0227
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A transgenic Leukocyte Ig-Like Receptor (LILR) mouse model useful for exploring the function, expression pattern, and developmental regulation of the human Ig-like activating and inhibitory receptor Leukocyte Ig-Like Receptor. The LILR transgenes were expressed in a similar, but not identical, pattern to that observed in humans.

This mouse model has been described in:

J Immunol. 2003, 171(6), 3056-63.

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Rat CD45 monoclonal antibody YTH 24.5

22 January, 2016 | Monoclonal hybridomas Code: AB65
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Rat CD45 monoclonal antibody YTH 54.12

22 January, 2016 | Monoclonal hybridomas Code: AB115
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Murid herpesvirus-4 (MuHV 4) Antibodies

15 January, 2016 | Monoclonal hybridomas Code: AB-0293 - AB-0366
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Influenza PR-8 Antibodies

15 January, 2016 | Monoclonal hybridomas Code: AB-0280 - AB-0292
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Stable human U2OS biotin-Ub cell line for ubiquitin research

22 June, 2015 | Cell lines Code: CL-0229
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A stable human U2OS biotin-Ub cell line expressing multiple copies of biotinylated ubiquitin under tight control by tetracycline. This cell line enables clean and easy identification and quantification of ubiquitin substrates, studies of ubiquitination mechanisms and ubiquitination inhibitor screens. Ubiquitination is thought to be a key signalling mechanism in diseases such as cancer and these cells will be a powerful tool for the ubiquitin research community.

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Recombinant human RAD51

22 June, 2015 | Recombinant proteins Code: OM-0215
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Large quantities of recombinant full-length human RAD51 protein useful for biochemical and biological assays including for DNA strand exchange, protein-protein/protein-DNA interaction studies in vitro and in vivo and Western blots.

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Macaca mulatta (Rhesus macaque)/ *Macaca fascicularis (Cynomolgus macaque) FcγRIIb expressing cell lines

23 April, 2015 | Cell lines Code: CL-0270
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Clone of CHO-K1 cells expressing * Macaca mulatta (Rhesus macaque) / *Macaca fascicularis (Cynomolgus macaque) FcγRIIb as a transmembrane protein.

*The sequence for the extracellular domains of FcγRIIb from Rhesus macaque (NP_001258577) and a predicted sequence for one polymorphic form of FcγRIIb from Cynomolgus macaque (NW_005092960) are identical at the amino acid level. There is one amino acid change (Leu104Met) relative to the Cynomolgus macaque sequence AAL92097.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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Papio anubis (Olive baboon) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell lines Code: CL-0239
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Clone of CHO-K1 cells expressing *Papio anubis (Olive baboon) FcγRIIIa anchored to glycosyl-phosphatydylinositiol.

*Sequences predicted from NCBI NM_001112647.1 are identical at the amino acid level.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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HeLa-Mitotrap cell lines

7 January, 2015 | Cell lines Code: CL-0272, CL-0273, CL-0274 and CL-0275
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HeLa-Mitotrap, HeLa-Mitotrap-Ap1g1-FKBP, HeLa-Mitotrap-Ap2a2-FKBP and HeLa-Mitotrap-GGA2-FKBP are stably transfected cell lines. HeLa-Mitotrap expresses a mitochondrial trapping construct with an FRB domain and is maintained in hygromycin-containing medium. The other three cell lines also express Mitotrap, and in addition they express a protein of interest with an FKBP domain attached, which can be trapped onto mitochondrial when rapamycin is added. The three proteins of interest are Ap1g1 or gamma-adaptin; Ap2a2 or alpha-adaptin isoform C; and GGA2.

These cell lines were described in:

Dev Cell, 2010, 18: 324–331

Curr Biol 2012, 22: 1711–1716

J Cell Sci 2013, 126: 2102–2113

Curr Protoc Cell Biol, 2013, 61: 15.20.11–17

Cambridge Enterprise Limited is the exclusive licensor for the HeLa-Mitotrap cell lines. We have partnered with ECACC (The European Collection of Authenticated Cell Cultures; a Public Health England Culture Collection) to manage distribution of these cell lines on our behalf. Enquires to either organisation are welcomed.

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BG4 anti-DNA/RNA G-quadruplex antibody (and related small molecules)

22 April, 2014 | Single-chain variable fragment Code: AB-0268
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BG4 anti-DNA/RNA G-quadruplex antibody is an engineered, structure-specific scFv probe that binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures as described in the following publications by Biffi, Antonio, Tannahill, McCafferty and Balasubramanian:

Nat. Chem. 5, 182–186 (2013)

Nat. Chem. 6, 75–80 (2014)

Angew. Chem. Int. Ed. Engl. 54, 910-913 (2015)

Nat. Genetics, 48,1267–1272 (2016)

The nucleic acid stabiliser molecules pyridostatin (PDS) and carboxy-pyridostatin described in the above and related papers are also available for licensing.

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FcgRllb 131R receptor expressing cell line

29 September, 2011 | Cell lines Code: CL-0206
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CHO cells transfected to express human FcgRllb allotype 131R as a transmembrane protein with its native intracellular domain (including a polymorphism in the transmembrane region). Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

Publications:

Armour et al. 2014. Eur J Immol. 44(3):905-14. PMID: 201343825.

Armour et al. 2014. PLoS One. 10;9(10). PMID: 25302805.

Harrison et al. 2012. J Pharm Biomed Anal. 7;63:23-8. PMID: 22366323.

Rohatgi et al. 2013. MBio. 27;4(5). PMID: 23982074.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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FcgRlla 131H receptor expressing cell line

29 September, 2011 | Cell lines Code: CL-0205
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CHO cells transfected to express human FcgRlla allotype 131H as a transmembrane protein with its native intracellular domain. Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

Publications:

Armour et al. 2014. Eur J Immol. 44(3):905-14. PMID: 201343825.

Armour et al. 2014. PLoS One. 10;9(10). PMID: 25302805.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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FcgRlla 131R receptor expressing cell line

29 September, 2011 | Cell lines Code: CL-0204
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CHO cells transfected to express human FcgRlla allotype 131R as a transmembrane protein with its native intracellular domain. Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

Publications:

Armour et al. 2014. Eur J Immol. 44(3):905-14. PMID: 201343825.

Armour et al. 2014. PLoS One. 10;9(10). PMID: 25302805.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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FcgRIIIa 158F/GPI receptor expressing cell line

29 September, 2011 | Cell lines Code: CL7
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CHO cells transfected to express human FcgRIIIa of the 158F allotype on their surface as glycosyl-phosphatidylinositol-anchored molecules useful for investigation of the binding of recombinant antibodies to human Fc receptors.

Publications:

Armour et al. 2010. J Immunol Methods. 31;354(1-2):20-33. PMID: 20138184.

Armour et al. 2014. Eur J Immol. 44(3):905-14. PMID: 201343825.

Armour et al. 2014. PLoS One. 10;9(10). PMID: 25302805.

Harrison et al. 2012. J Pharm Biomed Anal. 7;63:23-8. PMID: 22366323.

Rohatgi et al. 2013. MBio. 27;4(5). PMID: 23982074.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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FcgRIIIa 158V/GPI receptor expressing cell line

29 September, 2011 | Cell lines Code: CL6
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CHO cells transfected to express human FcgRIIIa of the 158V allotype on their surface as glycosyl-phosphatidylinositol-anchored molecules useful for investigation of the binding of recombinant antibodies to human Fc receptors.

Publications:

Armour et al. 2010. J Immunol Methods. 31;354(1-2):20-33. PMID: 20138184.

Armour et al. 2014. Eur J Immol. 44(3):905-14. PMID: 201343825.

Armour et al. 2014. PLoS One. 10;9(10). PMID: 25302805.

Harrison et al. 2012. J Pharm Biomed Anal. 7;63:23-8. PMID: 22366323.

Rohatgi et al. 2013. MBio. 27;4(5). PMID: 23982074.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

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Maltose binding protein polyclonal antibody

29 September, 2011 | Polyclonal antibodies Code: AB-0213
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Antibodies raised against maltose binding protein tag which are rabbit polyclonal antibodies of the IgG isotype. Has been used for western blotting, immunoprecipitation, immunocytochemistry and immunofluorescence studies.

Publications:

Wolf E et al. Exon, intron and splice site locations in the spliceosomal B complex. EMBO J 28:2283–2292 (2009). EM. PubMed: 19536130. http://www.nature.com/emboj/index.html

Bessonov S et al. Isolation of an active step I spliceosome and composition of its RNP core. Nature : (2008). WB. PubMed: 18322460. http://www.nature.com/nature/index.html

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