Available reagents

Licensing for the research community

Cell lines, antibodies, proteins, DNA constructs, small molecules and other research tools generated by scientists at the University of Cambridge play a key role in laboratory research. There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing, so please contact us directly with your specific needs.

Non-exclusive licences with standard terms and conditions are available for available reagents.

MOCHA Mouse Skin Fibroblast Cell Line

16 February, 2017 | Cell lines Code: CL-0385
Read more

MOCHA is a naturally occurring mouse mutant with a null mutation in the delta subunit of AP3 complex. In MOCHA cells, the ß3 and μ3 subunits co-assemble into a heterodimer whereas the σ3 subunit remains monomeric. The mouse has a similar phenotype to the human genetic disorder, Hermansky Pudlak Syndrome (HPS). In both mice and humans, AP3 deficiency results in hypopigmentation because of abnormalities in melanosomes. The MOCHA mouse skin fibroblast cell line can be used to investigate the assembly of AP3 complexes and AP3 function.

J. Cell Biol. 2002, 156, 327-336.

Make an Enquiryread more arrow

Enquiry for MOCHA Mouse Skin Fibroblast Cell Line

Reagents Enquiry

Mouse MAC-1 monoclonal antibody YBM 15.1.6

22 December, 2016 | Code: CL-0391
Read more

Hybridoma cell line producing YBM 15.1.6 antibodies raised against mouse MAC-1, the murine equivalent of CD11b. The YBM 15.1.6 antibodies are rat monoclonal antibodies of the IgG2b isotype.

Make an Enquiryread more arrow

Enquiry for Mouse MAC-1 monoclonal antibody YBM 15.1.6

Reagents Enquiry

Mouse MAC-1 monoclonal antibody YBM 6.1.10

22 December, 2016 | Monoclonal hybridomas Code: CL-0390
Read more

Hybridoma cell line producing YBM 6.1.10 antibodies raised against mouse MAC-1, the murine equivalent of CD11b. The YBM 6.1.10 antibodies are rat monoclonal antibodies of the IgG2a isotype.

Make an Enquiryread more arrow

Enquiry for Mouse MAC-1 monoclonal antibody YBM 6.1.10

Reagents Enquiry

Tetrabutylammonium (4’methyl-[2,2’bipyridin]-5yl)methanesulfonate

15 November, 2016 | Small molecules Code: OM-0384
Read more

Tetrabutylammonium (4’methyl-[2,2’bipyridin]-5yl)methanesulfonate is a small molecule that has been shown to act as a bifunctional ligand for iridium to control regioselectivity of iridium-catalysed borylation on cationic substrates as shown in the following publication:

J. Am. Chem. Soc. 2016, 138, 12759−12762

This purified small molecule is available for licensing. Please get in touch if you would like more information.

ligandjpg

Make an Enquiryread more arrow

Enquiry for Tetrabutylammonium (4’methyl-[2,2’bipyridin]-5yl)methanesulfonate

Reagents Enquiry

-5yl)methanesulfonate - Code: OM-0384']

E2_C6 scFv antibody raised against MT1-MMP14

20 October, 2016 | Single-chain variable fragment Code: AB-0381
Read more

The scFv-Fc antibody named E2_C6 raised against membrane-associated matrix metalloproteinase-14 (MT1-MMP14) was developed at the University of Cambridge. This antibody fragment to MT1-MMP14 potently and specifically inhibits its cell surface functions.

Publications:

Oncotarget (2016) 7(13):16773-167792

 

Make an Enquiryread more arrow

Enquiry for E2_C6 scFv antibody raised against MT1-MMP14

Reagents Enquiry

CRISPR Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

25 May, 2016 | Plasmids Code: Undisclosed
Read more

A CRISPR Cas9D10A-based screening approach that combines an All-in-One Cas9D10A nickase vector with fluorescence-activated cell sorting enrichment has been developed in the lab of Prof Steve Jackson at the University of Cambridge. The technique is combined with high-throughput genotypic and phenotypic clonal screening strategies to generate isogenic knockouts and knock-ins highly efficiently, which leads to minimal off-target effects. Please see the published paper describing the technology below:

Chiang, T.-W. W., le Sage, C., Larrieu, D., Demir, M. and Jackson, S. P. Sci Rep. 2016, 6, 24356.

Please get in touch if you would like more information.

Make an Enquiryread more arrow

Enquiry for CRISPR Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

Reagents Enquiry

Tephrosin

25 May, 2016 | Small molecules Code: OM-0379
Read more

Tephrosin is a plant-based rotenoid which has known anti-cancer properties. An academic group at the University of Cambridge has recently completed the novel chemical synthesis of Tephrosin. The purity of the product is in excess of 99% as confirmed by LC-MS and NMR.

Purified Tephrosin is available for licensing. Please get in touch if you would like more information.

Tephrosin ≥95% (LC/MS-ELSD)

Make an Enquiryread more arrow

Enquiry for Tephrosin

Reagents Enquiry

PC12 HD-Q23 (lines 14 & 7) and HD-Q74 (lines 10 & 1b) cell lines for Huntington’s Disease research

3 February, 2016 | Cell lines Code: CL4 and CL5 (Rub-1856-07)
Read more

Two stable inducible rat phaeochromocytoma (PC12) cell lines expressing GFP-tagged exon 1 of the huntingtin gene with either 23 or 74 glutamine repeats (PC12 HD-Q23 or PC12 HD-Q74), driven by a doxycycline-dependent Tet-On promoter – these cell lines can be used for in vitro studies to evaluate compounds to treat Huntington’s Disease.

Download more information here.

Publications:

Wyttenbach et al. (2001) Hum Mol Genet. 10,1829-45.

Make an Enquiryread more arrow

Enquiry for PC12 HD-Q23 (lines 14 & 7) and HD-Q74 (lines 10 & 1b) cell lines for Huntington’s Disease research

Reagents Enquiry

Leukocyte Ig-Like Receptor (LILR) Mouse Model

1 February, 2016 | Other Code: OM-0227
Read more

A transgenic Leukocyte Ig-Like Receptor (LILR) mouse model useful for exploring the function, expression pattern, and developmental regulation of the human Ig-like activating and inhibitory receptor Leukocyte Ig-Like Receptor. The LILR transgenes were expressed in a similar, but not identical, pattern to that observed in humans.

This mouse model has been described in:

J Immunol. 2003, 171(6), 3056-63.

Make an Enquiryread more arrow

Enquiry for Leukocyte Ig-Like Receptor (LILR) Mouse Model

Reagents Enquiry

Rat CD45 monoclonal antibody YTH 24.5

22 January, 2016 | Monoclonal hybridomas Code: AB65
Read more
Make an Enquiryread more arrow

Enquiry for Rat CD45 monoclonal antibody YTH 24.5

Reagents Enquiry

Rat CD45 monoclonal antibody YTH 54.12

22 January, 2016 | Monoclonal hybridomas Code: AB115
Read more
Make an Enquiryread more arrow

Enquiry for Rat CD45 monoclonal antibody YTH 54.12

Reagents Enquiry

Murid herpesvirus-4 (MuHV 4) Antibodies

15 January, 2016 | Monoclonal hybridomas Code: AB-0293 - AB-0366
Read more
Make an Enquiryread more arrow

Enquiry for Murid herpesvirus-4 (MuHV 4) Antibodies

Reagents Enquiry

Influenza PR-8 Antibodies

15 January, 2016 | Monoclonal hybridomas Code: AB-0280 - AB-0292
Read more
Make an Enquiryread more arrow

Enquiry for Influenza PR-8 Antibodies

Reagents Enquiry

Novel IgY purification protein

6 November, 2015 | Recombinant proteins Code: OM-0276
Read more

A novel chicken IgY purification protein that binds directly to chicken IgY antibodies with high affinity (ie. 590nM) is available for licensing. The protein was identified through a reverse protein capture approach using sepharose beads either conjugated to chicken IgY (whole molecule) or human IgG (Fc fragment). The protein has been successfully used as a chicken IgY precipitation reagent in immunoprecipitation assays.

The function of this protein is akin to that of mammalian TRIM21 and its interaction with IgG.

Make an Enquiryread more arrow

Enquiry for Novel IgY purification protein

Reagents Enquiry

KARPAS 707 Human Myeloma cell line

5 November, 2015 | Cell lines Code: CL19
Read more

A human plasmacytoma cell line (Karpas 707) useful in oncology research. The cultured cells are negative for Epstein-Barr viral nuclear antigen and free of mycoplasma. They are similar to plasma cells and secrete only lambda light chains. The cells are hypodiploid and contain the Philadelphia chromosome and other abnormalities.

Publications:

Karpas et al. (1982) Science, 216, 997-999

Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio.

Make an Enquiryread more arrow

Enquiry for KARPAS 707 Human Myeloma cell line

Reagents Enquiry

Stable human U2OS biotin-Ub cell line for ubiquitin research

22 June, 2015 | Cell lines Code: CL-0229
Read more

A stable human U2OS biotin-Ub cell line expressing multiple copies of biotinylated ubiquitin under tight control by tetracycline. This cell line enables clean and easy identification and quantification of ubiquitin substrates, studies of ubiquitination mechanisms and ubiquitination inhibitor screens. Ubiquitination is thought to be a key signalling mechanism in diseases such as cancer and these cells will be a powerful tool for the ubiquitin research community.

Download more information

Make an Enquiryread more arrow

Enquiry for Stable human U2OS biotin-Ub cell line for ubiquitin research

Reagents Enquiry

Recombinant human RAD51

22 June, 2015 | Recombinant proteins Code: OM-0215
Read more

Large quantities of recombinant full-length human RAD51 protein useful for biochemical and biological assays including for DNA strand exchange, protein-protein/protein-DNA interaction studies in vitro and in vivo and Western blots.

Make an Enquiryread more arrow

Enquiry for Recombinant human RAD51

Reagents Enquiry

Macaca mulatta (Rhesus macaque)/ *Macaca fascicularis (Cynomolgus macaque) FcγRIIb expressing cell lines

23 April, 2015 | Cell lines Code: CL-0270
Read more

Clone of CHO-K1 cells expressing * Macaca mulatta (Rhesus macaque) / *Macaca fascicularis (Cynomolgus macaque) FcγRIIb as a transmembrane protein.

*The sequence for the extracellular domains of FcγRIIb from Rhesus macaque (NP_001258577) and a predicted sequence for one polymorphic form of FcγRIIb from Cynomolgus macaque (NW_005092960) are identical at the amino acid level. There is one amino acid change (Leu104Met) relative to the Cynomolgus macaque sequence AAL92097.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Macaca mulatta (Rhesus macaque)/ *Macaca fascicularis (Cynomolgus macaque) FcγRIIb expressing cell lines

Reagents Enquiry

Macaca mulatta (Rhesus macaque) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell lines Code: CL-0238
Read more

Clone of CHO-K1 cells expressing Rhesus macaque (Macaca mulatta) FcγRIIIa anchored to glycosyl-phosphatydylinositiol. (Predicted from DQ423379 NCBI sequence)

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Macaca mulatta (Rhesus macaque) FcγRIIIa GPI expressing cell lines

Reagents Enquiry

Macaca fascicularis (Cynomolgus macaque) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell lines Code: CL-0237
Read more

Clone of CHO-K1 cells expressing Cynomolgus macaque (Macaca fascicularis) FcγRIIIa anchored to glycosyl-phosphatydylinositiol. (Predicted from AF485815 NCBI sequence)

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Macaca fascicularis (Cynomolgus macaque) FcγRIIIa GPI expressing cell lines

Reagents Enquiry

Papio anubis (Olive baboon) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell lines Code: CL-0239
Read more

Clone of CHO-K1 cells expressing *Papio anubis (Olive baboon) FcγRIIIa anchored to glycosyl-phosphatydylinositiol.

*Sequences predicted from NCBI NM_001112647.1 are identical at the amino acid level.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Papio anubis (Olive baboon) FcγRIIIa GPI expressing cell lines

Reagents Enquiry

Cercocebus torquatus (Red-crowned Mangabey) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell lines Code: CL-0236
Read more

Clone of CHO-K1 cells expressing *Cercocebus torquatus (Red-crowned Mangabey) FcγRIIIa anchored to glycosyl-phosphatydylinositiol (GPI).

*Sequences predicted from NCBI DQ423376.1 are identical at the amino acid level.

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Cercocebus torquatus (Red-crowned Mangabey) FcγRIIIa GPI expressing cell lines

Reagents Enquiry

Pan troglodytes (Chimpanzee) FcγRIIIa GPI receptor expressing cell line

23 April, 2015 | Cell lines Code: CL-0235
Read more

Clone of CHO-K1 cells expressing chimpanzee (Pan troglodytes) FcγRIIIa anchored to glycosyl-phosphatydylinositiol (GPI). (Predicted from XM_001174057.1 sequence)

Cambridge Enterprise Limited is the exclusive licensor this cell line. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for Pan troglodytes (Chimpanzee) FcγRIIIa GPI receptor expressing cell line

Reagents Enquiry

HeLa-Mitotrap cell lines

7 January, 2015 | Cell lines Code: CL-0272, CL-0273, CL-0274 and CL-0275
Read more

HeLa-Mitotrap, HeLa-Mitotrap-Ap1g1-FKBP, HeLa-Mitotrap-Ap2a2-FKBP and HeLa-Mitotrap-GGA2-FKBP are stably transfected cell lines. HeLa-Mitotrap expresses a mitochondrial trapping construct with an FRB domain and is maintained in hygromycin-containing medium. The other three cell lines also express Mitotrap, and in addition they express a protein of interest with an FKBP domain attached, which can be trapped onto mitochondrial when rapamycin is added. The three proteins of interest are Ap1g1 or gamma-adaptin; Ap2a2 or alpha-adaptin isoform C; and GGA2.

These cell lines were described in:

Dev Cell, 2010, 18: 324–331

Curr Biol 2012, 22: 1711–1716

J Cell Sci 2013, 126: 2102–2113

Curr Protoc Cell Biol, 2013, 61: 15.20.11–17

Cambridge Enterprise Limited is the exclusive licensor for the HeLa-Mitotrap cell lines. We have partnered with ECACC (The European Collection of Authenticated Cell Cultures; a Public Health England Culture Collection) to manage distribution of these cell lines on our behalf. Enquires to either organisation are welcomed.

Make an Enquiryread more arrow

Enquiry for HeLa-Mitotrap cell lines

Reagents Enquiry