Available reagents

Licensing for the research community

Cell lines, antibodies, proteins, DNA constructs, small molecules and other research tools generated by scientists at the University of Cambridge play a key role in laboratory research. There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing, so please contact us directly with your specific needs.

Non-exclusive licences with standard terms and conditions are available for selected reagents.

Stable human cell line for ubiquitin research

22 June, 2015 | Cell Lines Code: CL-0229
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A stable human cell line expressing multiple copies of biotinylated ubiquitin under tight control by tetracycline. This cell line enables clean and easy identification and quantification of ubiquitin substrates, studies of ubiquitination mechanisms and ubiquitination inhibitor screens. Ubiquitination is thought to be a key signalling mechanism in diseases such as cancer and these cells will be a powerful tool for the ubiquitin research community.

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Recombinant human RAD51

22 June, 2015 | Other Code: OM-0215
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Large quantities of recombinant full-length human RAD51 useful for biochemical and biological assays including for DNA strand exchange, protein-protein/protein-DNA interaction studies in vitro and in vivo and Western blots.

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Macaca mulatta (Rhesus macaque)/ *Macaca fascicularis (Cynomolgus macaque) FcγRIIb expressing cell lines

23 April, 2015 | Cell Lines Code: CL-0270
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Clone of CHO-K1 cells expressing * Macaca mulatta (Rhesus macaque) / *Macaca fascicularis (Cynomolgus macaque) FcγRIIb as a transmembrane protein.

*The sequence for the extracellular domains of FcγRIIb from Rhesus macaque (NP_001258577) and a predicted sequence for one polymorphic form of FcγRIIb from Cynomolgus macaque (NW_005092960) are identical at the amino acid level. There is one amino acid change (Leu104Met) relative to the Cynomolgus macaque sequence AAL92097.

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Macaca mulatta (Rhesus macaque) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell Lines Code: CL-0238
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Clone of CHO-K1 cells expressing Rhesus macaque (Macaca mulatta) FcγRIIIa anchored to glycosyl-phosphatydylinositiol. (Predicted from DQ423379 NCBI sequence)

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Macaca fascicularis (Cynomolgus macaque) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell Lines Code: CL-0237
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Clone of CHO-K1 cells expressing Cynomolgus macaque (Macaca fascicularis) FcγRIIIa anchored to glycosyl-phosphatydylinositiol. (Predicted from AF485815 NCBI sequence)

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Papio anubis (Olive baboon) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell Lines Code: CL-0239
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Clone of CHO-K1 cells expressing *Papio anubis (Olive baboon) FcγRIIIa anchored to glycosyl-phosphatydylinositiol.

*Sequences predicted from NCBI NM_001112647.1 are identical at the amino acid level.

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Cercocebus torquatus (Red-crowned Mangabey) FcγRIIIa GPI expressing cell lines

23 April, 2015 | Cell Lines Code: CL-0236
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Clone of CHO-K1 cells expressing *Cercocebus torquatus (Red-crowned Mangabey) FcγRIIIa anchored to glycosyl-phosphatydylinositiol.

*Sequences predicted from NCBI DQ423376.1 are identical at the amino acid level.

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Pan troglodytes (Chimpanzee) FcγRIIIa GPI receptor expressing cell line

23 April, 2015 | Cell Lines Code: CL-0235
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Clone of CHO-K1 cells expressing chimpanzee (Pan troglodytes) FcγRIIIa anchored to glycosyl-phosphatydylinositiol. (Predicted from XM_001174057.1 sequence)

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HeLa-Mitotrap cell lines

7 January, 2015 | Cell Lines Code: CL-0272, CL-0273, CL-0274 and CL-0275
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HeLa-Mitotrap, HeLa-Mitotrap-Ap1g1-FKBP, HeLa-Mitotrap-Ap2a2-FKBP and HeLa-Mitotrap-GGA2-FKBP are stably transfected cell lines. HeLa-Mitotrap expresses a mitochondrial trapping construct with an FRB domain and is maintained in hygromycin-containing medium. The other three cell lines also express Mitotrap, and in addition they express a protein of interest with an FKBP domain attached, which can be trapped onto mitochondrial when rapamycin is added. The three proteins of interest are Ap1g1 or gamma-adaptin; Ap2a2 or alpha-adaptin isoform C; and GGA2.

These cell lines were described in:

Dev Cell 18: 324–331

Curr Biol 22: 1711–1716

J Cell Sci 126: 2102–2113

Curr Protoc Cell Biol 61: 15.20.11–17

 

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BG4 DNA/RNA G-quadruplex antibody (and related small molecules)

22 April, 2014 | Single-chain variable fragment Code: AB-0268
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An engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures as described in the following publications by Biffi, Antonio, Tannahill, McCafferty and Balasubramanian:

Nat. Chem. 5, 182–186 (2013)

Nat. Chem. 6, 75–80 (2014)

Angew. Chem. Int. Ed. Engl. 54, 910-913 (2015)

The nucleic acid stabiliser molecules pyridostatin (PDS) and carboxy-pyridostatin described in the above and related papers are also available for licensing.

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Human alpha synuclein cell lines

29 November, 2012 | Cell Lines Code: CL-0240, CL-0241 and CL-0242
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PC12 Tet-On cells expressing human α-synuclein (HA and his tagged); one cell line expresses wild-type α-synuclein and two other cell lines express one of two mutant forms of human α-synuclein, either A53T or A30P. Both of these two mutations are responsible for certain rare familial forms of Parkinson’s disease.

These cells are first described in:

Alpha-Synuclein is degraded by both autophagy and the proteasome. Webb JL et al, J Biol Chem (2003) 278 (27):25009

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Recombinant human RAD51 protein

29 September, 2011 | Other Code: OM0215
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Recombinant full length human RAD51 protein purified from bacteria. Useful in biochemical and biological assays, for example, for research into DNA repair by homologous recombination by performing DNA strand-exchange assays, and may also be used in protein–protein and protein–DNA interaction studies both in vitro and in vivo.

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FcgRllb 131R receptor expressing cell line

29 September, 2011 | Cell Lines Code: CL206
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CHO cells transfected to express human FcgRllb allotype 131R as a transmembrane protein with its native intracellular domain (including a polymorphism in the transmembrane region). Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

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FcgRlla 131H receptor expressing cell line

29 September, 2011 | Cell Lines Code: CL205
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CHO cells transfected to express human FcgRlla allotype 131H as a transmembrane protein with its native intracellular domain. Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

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FcgRlla 131R receptor expressing cell line

29 September, 2011 | Cell Lines Code: CL204
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CHO cells transfected to express human FcgRlla allotype 131R as a transmembrane protein with its native intracellular domain. Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

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FcgRIIIa 158F/GPI receptor expressing cell line

29 September, 2011 | Cell Lines Code: CL7
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CHO cells transfected to express human FcyRIIIa of the 158F allotype on their surface as glycosyl-phosphatidylinositol-anchored molecules useful for investigation of the binding of recombinant antibodies to human Fc receptors.

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FcgRIIIa 158V/GPI receptor expressing cell line

29 September, 2011 | Cell Lines Code: CL6
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CHO cells transfected to express human FcyRIIIa of the 158V allotype on their surface as glycosyl-phosphatidylinositol-anchored molecules useful for investigation of the binding of recombinant antibodies to human Fc receptors.

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Maltose binding protein polyclonal antibody

29 September, 2011 | Polyclonal antibodies Code: AB0213
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Antibodies raised against maltose binding protein tag which are rabbit polyclonal antibodies of the IgG isotype. Has been used for western blotting, immunoprecipitation, immunocytochemistry and immunofluorescence studies.

Publications:

Wolf E et al. Exon, intron and splice site locations in the spliceosomal B complex. EMBO J 28:2283–2292 (2009). EM. PubMed: 19536130. http://www.nature.com/emboj/index.html

Bessonov S et al. Isolation of an active step I spliceosome and composition of its RNP core. Nature : (2008). WB. PubMed: 18322460. http://www.nature.com/nature/index.html

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Glutathione-S-Transferase (GST) polyclonal antibody

29 September, 2011 | Polyclonal antibodies Code: AB0212
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Antibodies raised against glutathione-S-transferase from Schistosoma japonicum which are rabbit polyclonal antibodies of the IgG isotype. Has been used for ELISA, westen blotting, immunoprecipitation, immunocytochemistry and immunofluorescence studies.

Publications:

Kalamajski S. & Oldberg A. Homologous Sequence in Lumican and Fibromodulin Leucine-rich Repeat 5–7 Competes for Collagen Binding. J Biol Chem 284:534-9 (2009). I-ELISA. PubMed: 19008226. www.jbc.org/

Bansal P.K. et al. Sgt1 dimerization is negatively regulated by protein kinase CK2-mediated phosphorylation at S361. J Biol Chem : (2009). WB. PubMed: 19398558. www.jbc.org/

Kathiresan T. et al. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics 8:1972–87 (2009). PubMed: 19423573. www.mcponline.org/

Scales T.M. et al. Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. J Cell Sci 122:2424–35 (2009). WB. PubMed: 19549690. jcs.biologists.org/

Wood-Kaczmar A. et al. An alternatively spliced form of glycogen synthase kinase-3beta is targeted to growing neurites and growth cones. Mol Cell Neurosci 42:184–94 (2009). WB; Rat. PubMed: 19607922. www.sciencedirect.com/science/journal/10447431

Jones B.W. & Hinkle P.M. Subcellular trafficking of the TRH receptor: effect of phosphorylation. Mol Endocrinol 23:1466–78 (2009). PubMed: 19541745. mend.endojournals.org/

Matheson L.A. et al. Correct targeting of plant ARF GTPases relies on distinct protein domains. Traffic 9:103–20 (2008). WB. PubMed: 17988226. www.traffic.dk/

Bossard C. et al. Dimeric PKD regulates membrane fission to form transport carriers at the TGN. J Cell Biol 179:1123–31 (2007). PubMed: 18086912. jcb.rupress.org/

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Herpes Simplex Virus Type 1 (HSV-1) pUL37 monoclonal antibody CB8

29 September, 2011 | Monoclonal hybridomas Code: AB0225
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Hybridoma cell line producing CB8 antibodies raised against Herpes Simplex Virus Type-1 protein pUL37, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

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Herpes Simplex Virus Type 1 (HSV-1) pUL36 (VP1/2) monoclonal antibody CB4

29 September, 2011 | Monoclonal hybridomas Code: AB0224
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Hybridoma cell line producing CB4 antibodies raised against Herpes Simplex Virus Type-1 protein pUL36 (VP1/2), which are mouse monoclonal antibodies of the IgG2b isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

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Herpes Simplex Virus Type 1 (HSV-1) VP21/VP22a (scaffold) monoclonal antibody LP13

29 September, 2011 | Monoclonal hybridomas Code: AB-0227
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Hybridoma cell line producing LP13 antibodies raised against Herpes Simplex Virus Type-1 VP21/VP22a scaffold protein, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

A non-exclusive licence with standard terms and conditions is available for this reagent.

To obtain a licence
1. Download the licence document and enter your company name and full registered address on page 1 of licence agreement.
2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

Research Reagents
Cambridge Enterprise Limited
University of Cambridge
The Hauser Forum
3 Charles Babbage Road
Cambridge CB3 0GT

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Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein H monoclonal antibody BBH1

29 September, 2011 | Monoclonal hybridomas Code: AB0223
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Hybridoma cell line producing BBH1 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein H, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot (no other anti-gH antibody detects protein by western blot).

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Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein B monoclonal antibody CB24

29 September, 2011 | Monoclonal hybridomas Code: AB0226
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Hybridoma cell line producing CB24 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein B, which are mouse monoclonal antibodies of the IgG2b isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

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