Hybridoma cell line producing AP12 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG1 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.
Minson A.C., Hodgman T.C., Digard P., Hancock D.C., Bell S.E., Buckmaster E.A.
An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
J Gen Virol. 1986 67:1001–13.