HeLa-Mitotrap cell lines

Code: CL-0272, CL-0273, CL-0274 and CL-0275

HeLa-Mitotrap, HeLa-Mitotrap-Ap1g1-FKBP, HeLa-Mitotrap-Ap2a2-FKBP and HeLa-Mitotrap-GGA2-FKBP are stably transfected cell lines. HeLa-Mitotrap expresses a mitochondrial trapping construct with an FRB domain and is maintained in hygromycin-containing medium. The other three cell lines also express Mitotrap, and in addition they express a protein of interest with an FKBP domain attached, which can be trapped onto mitochondrial when rapamycin is added. The three proteins of interest are Ap1g1 or gamma-adaptin; Ap2a2 or alpha-adaptin isoform C; and GGA2.

These cell lines were described in:

Dev Cell, 2010, 18: 324–331

Curr Biol 2012, 22: 1711–1716

J Cell Sci 2013, 126: 2102–2113

Curr Protoc Cell Biol, 2013, 61: 15.20.11–17

Cambridge Enterprise Limited is the exclusive licensor for the HeLa-Mitotrap cell lines. We have partnered with ECACC (The European Collection of Authenticated Cell Cultures; a Public Health England Culture Collection) to manage distribution of these cell lines on our behalf. Enquires to either organisation are welcomed.

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