HeLa-Mitotrap, HeLa-Mitotrap-Ap1g1-FKBP, HeLa-Mitotrap-Ap2a2-FKBP and HeLa-Mitotrap-GGA2-FKBP are stably transfected cell lines. HeLa-Mitotrap expresses a mitochondrial trapping construct with an FRB domain and is maintained in hygromycin-containing medium. The other three cell lines also express Mitotrap, and in addition they express a protein of interest with an FKBP domain attached, which can be trapped onto mitochondrial when rapamycin is added. The three proteins of interest are Ap1g1 or gamma-adaptin; Ap2a2 or alpha-adaptin isoform C; and GGA2.
These cell lines were described in:
Cambridge Enterprise Limited is the exclusive licensor for the HeLa-Mitotrap cell lines. We have partnered with ECACC (The European Collection of Authenticated Cell Cultures; a Public Health England Culture Collection) to manage distribution of these cell lines on our behalf. Enquires to either organisation are welcomed.Make an Enquiry