CRISPR Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing

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A CRISPR Cas9D10A-based screening approach that combines an All-in-One Cas9D10A nickase vector with fluorescence-activated cell sorting enrichment has been developed in the lab of Prof Steve Jackson at the University of Cambridge. The technique is combined with high-throughput genotypic and phenotypic clonal screening strategies to generate isogenic knockouts and knock-ins highly efficiently, which leads to minimal off-target effects. Please see the published paper describing the technology below:

Chiang, T.-W. W., le Sage, C., Larrieu, D., Demir, M. and Jackson, S. P. Sci Rep. 2016, 6, 24356.

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