Available reagents

Licensing for the research community

Cell lines, antibodies, proteins, DNA constructs, small molecules and other research tools generated by scientists at the University of Cambridge play a key role in laboratory research. There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing, so please contact us directly with your specific needs.

Non-exclusive licences with standard terms and conditions are available for available reagents.

Soybean clathrin heavy-chain monoclonal antibody 4A8

19 September, 2011 | Monoclonal hybridomas Code: AB50
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Hybridoma cell line producing 4A8 antibodies raised against soybean clathrin heavy chain which are mouse monoclonal antibodies of the IgG1 isotype. Useful for the study of clathrin distribution in a variety of plant cell systems including Arabidopsis and tobacco. Has been used for western blotting, immunofluorescence and immunoelectron microscopy.

A non-exclusive licence with standard terms and conditions is available for this reagent.

Publications:

Blackbourn, H.D. and Jackson, A.P. Plant clathrin heavy chain: sequence analysis and restricted localisation in growing pollen tubes. J. Cell Sci. 109, 777–787 (1996). jcs.biologists.org/

Lam, B.C.H., Sage, T.L., Bianchi, F. and Blumwald, E. Role of SH3 domain-containing proteins in clathrin-mediated vesicle trafficking in Arabiopsis. The Plant Cell. 13, 2499–2512 (2001). www.plantcell.org/

Lam, B.C.H., Sage, T.L., Bianchi, F. and Blumwald, E. Regulation of ADL6 activity by its associated molecular network. The Plant Journal 31, 565–576 (2002). onlinelibrary.wiley.com/journal/

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Human muscle creatine kinase monoclonal antibody 15CH2 (anti-human CK-MM)

19 September, 2011 | Monoclonal hybridomas Code: AB18
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Hybridoma cell line producing 15CH2 antibodies raised against human muscle creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction.

A non-exclusive licence with standard terms and conditions is available for this reagent.

Publications:

Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157–1162, 1984. www.clinchem.org/

Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310–315, 1985

Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236–1241, 1986. www.ajconline.org/

Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects–incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476–481, 1986. www.clinchem.org/

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Human brain creatine kinase monoclonal antibody 2AC5 (anti human CK-BB)

19 September, 2011 | Monoclonal hybridomas Code: AB17
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Hybridoma cell line producing 2AC5 antibodies raised against human brain creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction and potentially useful as a marker for head injuries, some neurological disorders and certain malignancies. Can also be used to generate a specific and sensitive assay for brain creatine kinase.

 

Publications:

Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157–1162, 1984. www.clinchem.org/

Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310–315, 1985.

Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236–1241, 1986. www.ajconline.org

Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects–incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476–481, 1986. www.clinchem.org/

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) VP16 (tegument) monoclonal antibody LP1 (anti-HSV VP16)

19 September, 2011 | Monoclonal hybridomas Code: AB107
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Hybridoma cell line producing LP1 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 tegument protein VP16, which are mouse monoclonal antibodies of the IgG1 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot. VP16 is a transactivator present in the HSV virion. The linear epitope lies within the N-terminal 24 residues. This is the only known monoclonal antibody to VP16.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Major Capsid antigen (VP5) monoclonal antibody LP12 (anti-HSV VP5)

19 September, 2011 | Monoclonal hybridomas Code: AB104
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Hybridoma cell line producing LP12 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 Major Capsid antigen (VP5), which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in western blots.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein G monoclonal antibody AP1 (anti-HSV gG)

19 September, 2011 | Monoclonal hybridomas Code: AB100
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Hybridoma cell line producing AP1 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein G monoclonal antibody LP10 (anti-HSV gG)

19 September, 2011 | Monoclonal hybridomas Code: AB94
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Hybridoma cell line producing LP10 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG3 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein E monoclonal antibody AP10 (anti-HSV gE)

19 September, 2011 | Monoclonal hybridomas Code: AB101
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Hybridoma cell line producing AP10 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein E, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

 

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Glycoprotein D monoclonal antibody LP2 (anti-HSV gD)

19 September, 2011 | Monoclonal hybridomas Code: AB88
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Hybridoma cell line producing LP2 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG2a isotype. Strongly neutralises virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.

Publications:

Minson A.C., Hodgman T.C., Digard P., Hancock D.C., Bell S.E., Buckmaster E.A.
An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
J Gen Virol. 1986 67:1001–13.
http://vir.sgmjournals.org/

Buckmaster E.A., Cranage M.P., McLean C.S., Coombs R.R., Minson A. The use of monoclonal antibodies to differentiate isolates of herpes simplex types 1 and 2 by neutralisation and reverse passive haemagglutination tests.
J Med Virol. 1984 13:193–202.
http://eu.wiley.com/WileyCDA/WileyTitle/productCd-JMV.html

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein C monoclonal antibody LP6 (anti-HSV gC)

19 September, 2011 | Monoclonal hybridomas Code: AB102
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Hybridoma cell line producing LP6 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein C, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

 

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Mouse CD45 monoclonal antibody YW 62.3.20

19 September, 2011 | Monoclonal hybridomas Code: AB62
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Hybridoma cell line producing YW 62.3.20 antibodies raised against mouse CD45 which are rat monoclonal antibodies of the IgG2b isotype.

 

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Mouse CD5 (lyt-1) monoclonal antibody YTS 121.5.2

19 September, 2011 | Monoclonal hybridomas Code: AB12
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Hybridoma cell line producing YTS 121.5.2 antibodies raised against mouse CD5 (lyt-1) which are rat monoclonal antibodies of the IgG2b isotype. Has been used in in vivo T cell depleting and flow cytometry studies.

 

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Human beta 2-microglobulin monoclonal antibody YTH 470.5

19 September, 2011 | Monoclonal hybridomas Code: AB81
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Hybridoma cell line producing YTH470.5 antibodies raised against human beta 2-microglobulin which are rat monoclonal antibodies of the IgG2b isotype.

Publications:

Eur J Immunol (1988) 18:1507–1514

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Herpes simplex virus type 1 strain SC16 thymidine kinase deletion mutant

5 September, 2011 | Other Code: OM8
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Human herpes simplex virus type 1 strain SC16 thymidine kinase deletion mutant, developed at the University of Cambridge. Useful in the study of antiviral drug resistance and the pathogenesis of thymidine kinase virus mutants.

Publications:

Journal of General Virology (1989) 70: 869–879. vir.sgmjournals.org/

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Human Serum-Resistance Associated Antigen

5 September, 2011 | Other Code: OM7
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Human Serum-Resistance Associated Antigen, potentially useful in the development of serodiagnostic for human infection with Tyrpanosoma brucei rhodesiense.

Publications:

Trends in Parasitology, 2004 20, 11, 519–523. http://www.cell.com/trends/parasitology/home

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ISG 75 Antigen (Invarient surface glycoprotein)

5 September, 2011 | Other Code: OM6
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Invarient surface glycoprotein (ISG 75) Antigen is a recombinant protein produced in E. coli corresponding to the extracellular domain of ISG75. Soluble to >5 mg/ml in the absence of detergents. Potentially useful in the development of a serodiagnostic for human or animal infection with Tyrpanosoma bruei.

Publications:

Trends in Parasitology, Vol 20, No 11 p519 Nov 2004. http://www.cell.com/trends/parasitology/

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ISG 65 Antigen (Invarient surface glycoprotein)

5 September, 2011 | Other Code: OM5
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Invarient surface glycoprotein (ISG 65) Antigen is a recombinant protein produced in E. coli corresponding to the extracellular domain of ISG65. Potentially useful in the development of a serodiagnostic for human or animal infection with Trypanosoma brucei.

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ISG 64 Antigen (invarient surface glycoprotein)

5 September, 2011 | Other Code: OM4
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Invarient surface glycoprotein (ISG 64) Antigen is a recombinant protein produced in E. coli corresponding to the extracellular domain of ISG64. Soluble to >5 mg/ml in the absence of detergents. Potentially useful in the development of a serodiagnostic for human or animal infection with Trypanosoma brucei.

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Recombinant mature form of Xenopus activin B growth factor produced in E. coli

5 September, 2011 | Recombinant proteins Code: OM2
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Recombinant mature form of Xenopus activin B growth factor produced in E. coli. It is a disulphide linked dimer with mass of 25kDa and homogenious by SDS polyacrylamide gel electrophoresis. Useful in the regulation of hematopoiesis, reproductive physiology and bone development.

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E7 protein of human papillomavirus 16 monoclonal antibody CamVir-4

1 September, 2011 | Monoclonal hybridomas Code: AB57
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Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 16-13

1 September, 2011 | Monoclonal hybridomas Code: AB37
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Hybridoma cell line producing IGF-1R 16-13 antibodies raised against the human insulin-like growth factor receptor (epitope 62-184) which are mouse monoclonal antibodies of the IgG2b isotype. Has been shown to enhance IGF binding and has been used for immunoprecipitation.

Publications:

J Biol Chem (1992) 267, 12955. www.jbc.org/

J Biol Chem (1993) 268, 1087. www.jbc.org/

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Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 17-69

1 September, 2011 | Cell lines Code: AB38
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Hybridoma cell line producing IGF-1R 17-69 antibodies raised against the human insulin-like growth factor receptor (epitope 440-586) which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause 35–60% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

Publications:

J Biol Chem (1992) 267, 12955. www.jbc.org/

J Biol Chem (1993) 268, 1087. www.jbc.org/

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Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 24-55

1 September, 2011 | Monoclonal hybridomas Code: AB33
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Hybridoma cell line producing IGF-1R 24-55 antibodies raised against the human insulin-like growth factor receptor (epitope 440-586) which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause 35–60% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

Publications:

J Biol Chem (1992) 267, 12955. www.jbc.org/

J Biol Chem (1993) 268, 1087. www.jbc.org/

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Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 24-57

1 September, 2011 | Monoclonal hybridomas Code: AB34
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Hybridoma cell line producing IGF-1R 24-57 antibodies raised against the human insulin-like growth factor receptor, which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause >80% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

Publications:

J Biol Chem (1992) 267, 12955. www.jbc.org/

J Biol Chem (1993) 268, 1087. www.jbc.org/

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