Available reagents

Licensing for the research community

Cell lines, antibodies, proteins, DNA constructs, small molecules and other research tools generated by scientists at the University of Cambridge play a key role in laboratory research. There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing, so please contact us directly with your specific needs.

Non-exclusive licences with standard terms and conditions are available for available reagents.

Human CD11a monoclonal antibody YTH 81.5

19 September, 2011 | Monoclonal hybridomas Code: AB82
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Hybridoma cell line producing YTH 81.5 antibodies raised against human CD11a which are rat monoclonal antibodies of the IgG2a isotype.

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Human CD7 monoclonal antibody YTH 30.15

19 September, 2011 | Monoclonal hybridomas Code: AB69
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Hybridoma cell line producing YTH 30.15 antibodies raised against human CD7 which are rat monoclonal antibodies of the IgG2b isotype.

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Mouse Thy-1 monoclonal antibody YTS 154.7.7.10

19 September, 2011 | Monoclonal hybridomas Code: AB10
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Hybridoma cell line producing YTS 154.7.7.10 antibodies raised against mouse Thy-1 which are rat monoclonal antibodies of the IgG2b isotype. Has been used in T+NK cell depletion in vivo and flow cytometry studies.

Publications:

Journal of Immunology Vol 134 5 May 1985 pages 3056–3061

Cobbold S. & H. Waldmann (1986) Transplantation 41, 634

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Mouse heat stable antigen monoclonal antibody YBM 5.10.4

19 September, 2011 | Monoclonal hybridomas Code: AB72
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Hybridoma cell line producing YBM 5.10.4 antibodies raised against mouse heat stable antigen which are rat monoclonal antibodies of the IgG2c isotype.

Publications:

Journal of Immunology Vol 134 5 May 1985 pages 3056–3061

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Mouse CD71 monoclonal antibody YTA 74.4.4

19 September, 2011 | Monoclonal hybridomas Code: AB110
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Hybridoma cell line producing YTA 74.4.4 antibodies raised against mouse CD71 which are monoclonal antibodies of the IgG2a isotype. Has been used in CD4 subset depletion in vivo and flow cytometry studies.

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Mouse CD18 monoclonal antibody YTS 213.1.1

19 September, 2011 | Monoclonal hybridomas Code: AB109
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Hybridoma cell line producing YTS 213.1.1 antibodies raised against mouse CD18 which are rat monoclonal antibodies of the IgG2a isotype.

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Mouse CD8 (lyt-3) monoclonal antibody YTS 156.7.7

19 September, 2011 | Monoclonal hybridomas Code: AB15
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Hybridoma cell line producing YTS 156.7.7 antibodies raised against mouse CD8 (lyt-3) which are rat monoclonal antibodies of the IgG2b isotype. Has been used in CD8 subset depletion in vivo and flow cytometry studies.

Publications:

Qin, S., Cobbold, S., Bemjamin, R., & Waldmann, H.

J. Exp. Med. 169, 779–794 (1989) http://jem.rupress.org/

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Mouse CD8 (lyt-2) monoclonal antibody YTS 105.18.10

19 September, 2011 | Monoclonal hybridomas Code: AB112
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Hybridoma cell line producing YTS 105.18.10 antibodies raised against mouse CD8 (lyt-2) which are rat monoclonal antibodies of the IgG2a isotype.

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Mouse CD4 epitope B monoclonal antibody YTA 3.1.2

19 September, 2011 | Monoclonal hybridomas Code: AB13
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Hybridoma cell line producing YTA 3.1.2 antibodies raised against mouse CD4 Epitope B which are rat monoclonal antibodies of the IgG2b isotype. Has been used in CD4 subset depletion in vivo and flow cytometry studies.

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Mouse CD4 epitope A monoclonal antibody YTS 177.9.6.1

19 September, 2011 | Monoclonal hybridomas Code: AB111
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Hybridoma cell line producing YTS 177.9.6.1 antibodies raised against mouse CD4 epitope A which are rat monoclonal antibodies of the IgG2a isotype.

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Anti-4E3 antibody to human vascular endothelial growth factor A

19 September, 2011 | Monoclonal hybridomas Code: AB84
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Hybridoma cell line producing anti-4E3 antibodies raised against human vascular endothelial growth factor A (VEGF-A) which are mouse monoclonal antibodies of the IgG1 isotype. This antibody has been used for both in vitro and in vivo neutralisation assays.

Selected publications:

Sharkey, A. M. et al. Novel antiangiogenic agents for use in contraception (2005). Contraception 71, 263–271.

Sengupta, J. et al. Immunoneutralisation of vascular endothelial growth factor inhibits pregnancy establishment in the rhesus monkey (Macaca mulatta) (2007). Reproduction 133(6), 1199-1211.

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CD19 membrane protein of mouse B lymphocytes monoclonal antibody 6D5

19 September, 2011 | Monoclonal hybridomas Code: AB9
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Hybridoma cell line producing 6D5 antibodies rasied against CD19 membrane protein of mouse B lymphocytes which are rat monoclonal antibodies of the IgG2a isotype.

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E7 protein of human papillomavirus 16 monoclonal antibody CamVir-3

19 September, 2011 | Monoclonal hybridomas Code: AB56
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Soybean clathrin heavy-chain monoclonal antibody 4A8

19 September, 2011 | Monoclonal hybridomas Code: AB50
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Hybridoma cell line producing 4A8 antibodies raised against soybean clathrin heavy chain which are mouse monoclonal antibodies of the IgG1 isotype. Useful for the study of clathrin distribution in a variety of plant cell systems including Arabidopsis and tobacco. Has been used for western blotting, immunofluorescence and immunoelectron microscopy.

A non-exclusive licence with standard terms and conditions is available for this reagent.

Publications:

Blackbourn, H.D. and Jackson, A.P. Plant clathrin heavy chain: sequence analysis and restricted localisation in growing pollen tubes. J. Cell Sci. 109, 777–787 (1996). jcs.biologists.org/

Lam, B.C.H., Sage, T.L., Bianchi, F. and Blumwald, E. Role of SH3 domain-containing proteins in clathrin-mediated vesicle trafficking in Arabiopsis. The Plant Cell. 13, 2499–2512 (2001). www.plantcell.org/

Lam, B.C.H., Sage, T.L., Bianchi, F. and Blumwald, E. Regulation of ADL6 activity by its associated molecular network. The Plant Journal 31, 565–576 (2002). onlinelibrary.wiley.com/journal/

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Human muscle creatine kinase monoclonal antibody 15CH2 (anti-human CK-MM)

19 September, 2011 | Monoclonal hybridomas Code: AB18
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Hybridoma cell line producing 15CH2 antibodies raised against human muscle creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction.

A non-exclusive licence with standard terms and conditions is available for this reagent.

Publications:

Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157–1162, 1984. www.clinchem.org/

Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310–315, 1985

Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236–1241, 1986. www.ajconline.org/

Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects–incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476–481, 1986. www.clinchem.org/

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Human brain creatine kinase monoclonal antibody 2AC5 (anti human CK-BB)

19 September, 2011 | Monoclonal hybridomas Code: AB17
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Hybridoma cell line producing 2AC5 antibodies raised against human brain creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction and potentially useful as a marker for head injuries, some neurological disorders and certain malignancies. Can also be used to generate a specific and sensitive assay for brain creatine kinase.

 

Publications:

Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157–1162, 1984. www.clinchem.org/

Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310–315, 1985.

Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236–1241, 1986. www.ajconline.org

Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects–incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476–481, 1986. www.clinchem.org/

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) VP16 (tegument) monoclonal antibody LP1 (anti-HSV VP16)

19 September, 2011 | Monoclonal hybridomas Code: AB107
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Hybridoma cell line producing LP1 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 tegument protein VP16, which are mouse monoclonal antibodies of the IgG1 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot. VP16 is a transactivator present in the HSV virion. The linear epitope lies within the N-terminal 24 residues. This is the only known monoclonal antibody to VP16.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Major Capsid antigen (VP5) monoclonal antibody LP12 (anti-HSV VP5)

19 September, 2011 | Monoclonal hybridomas Code: AB104
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Hybridoma cell line producing LP12 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 Major Capsid antigen (VP5), which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in western blots.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein G monoclonal antibody AP1 (anti-HSV gG)

19 September, 2011 | Monoclonal hybridomas Code: AB100
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Hybridoma cell line producing AP1 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein G monoclonal antibody LP10 (anti-HSV gG)

19 September, 2011 | Monoclonal hybridomas Code: AB94
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Hybridoma cell line producing LP10 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG3 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by western blot.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein E monoclonal antibody AP10 (anti-HSV gE)

19 September, 2011 | Monoclonal hybridomas Code: AB101
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Hybridoma cell line producing AP10 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein E, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

A non-exclusive licence with standard terms and conditions is available for this reagent.

 

 

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Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Glycoprotein D monoclonal antibody LP2 (anti-HSV gD)

19 September, 2011 | Monoclonal hybridomas Code: AB88
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Hybridoma cell line producing LP2 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG2a isotype. Strongly neutralises virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.

Publications:

Minson A.C., Hodgman T.C., Digard P., Hancock D.C., Bell S.E., Buckmaster E.A.
An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
J Gen Virol. 1986 67:1001–13.
http://vir.sgmjournals.org/

Buckmaster E.A., Cranage M.P., McLean C.S., Coombs R.R., Minson A. The use of monoclonal antibodies to differentiate isolates of herpes simplex types 1 and 2 by neutralisation and reverse passive haemagglutination tests.
J Med Virol. 1984 13:193–202.
http://eu.wiley.com/WileyCDA/WileyTitle/productCd-JMV.html

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Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein C monoclonal antibody LP6 (anti-HSV gC)

19 September, 2011 | Monoclonal hybridomas Code: AB102
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Hybridoma cell line producing LP6 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein C, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

 

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Mouse CD45 monoclonal antibody YW 62.3.20

19 September, 2011 | Monoclonal hybridomas Code: AB62
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Hybridoma cell line producing YW 62.3.20 antibodies raised against mouse CD45 which are rat monoclonal antibodies of the IgG2b isotype.

 

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