Reagents

Cell lines, antibodies, proteins, DNA constructs and other reagents generated by researchers are vital to laboratory research. Their availability is vital for the research community.

Licensing reagents for the research community

We license research reagents produced at the University of Cambridge to the wider research community.

For industry

There is a wide range of research reagents available for commercial licensing through Cambridge Enterprise. The selection is constantly changing so please contact us directly with your specific requirements.

Non-exclusive licences with standard terms and conditions are available for selected reagents.

  • BG4 DNA/RNA G-quadruplex antibody (and related small molecules)

    Code: AB-0268

    | Single-chain variable fragment

    An engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures as described in the following publications by Biffi, Antonio, Tannahill, McCafferty and Balasubramanian:

    Nat. Chem. 5, 182-186 (2013)

    Nat. Chem. 6, 75-80 (2014)

    The nucleic acid stabiliser molecules pyridostatin (PDS) and carboxy-pyridostatin described in the above and related papers are also available for licensing.

  • Human alpha synuclein cell lines

    Code: CL-0240, CL-0241, CL-0242

    | Cell lines

    PC12 Tet-On cells expressing human α-synuclein (HA and his tagged); one cell line expresses wild-type α-synuclein and two other cell lines express one of two mutant forms of human α-synuclein, either A53T or A30P. Both of these two mutations are responsible for certain rare familial forms of Parkinson’s disease.

    These cells are first described in: Alpha-Synuclein is degraded by both autophagy and the proteasome. Webb JL et al, J Biol Chem (2003) 278 (27):25009.

  • Recombinant Human RAD51 protein

    Code: OM0215

    | Other | View on Flintbox

    Recombinant full length human RAD51 protein purified from bacteria. Useful in biochemical and biological assays for example for research into DNA repair by homologous recombination by performing DNA strand-exchange assays and may also be used in protein-protein and protein-DNA interaction studies both in vitro and in vivo.

  • FcgRllb 131R receptor expressing cell line

    Code: CL206

    | Cell lines | View on Flintbox

    CHO cells transfected to express human FcgRllb allotype 131R as a transmembrane protein with its native intracellular domain (including a polymorphism in the transmembrane region). Useful for investigation of the binding of recombinant antibodies to human Fc receptors.

  • Maltose binding protein polyclonal antibody

    Code: AB0213

    | Polyclonal antibodies | View on Flintbox

    Antibodies raised against maltose binding protein tag which are rabbit polyclonal antibodies of the IgG isotype. Has been used for western blotting, immunoprecipitation, immunocytochemistry and immunofluorescence studies.

    Publications:

    Wolf E et al. Exon, intron and splice site locations in the spliceosomal B complex. EMBO J 28:2283-2292 (2009). EM. PubMed: 19536130. http://www.nature.com/emboj/index.html

    Bessonov S et al. Isolation of an active step I spliceosome and composition of its RNP core. Nature : (2008). WB. PubMed: 18322460. http://www.nature.com/nature/index.html

  • Glutathione-S-Transferase (GST) polyclonal antibody

    Code: AB0212

    | Polyclonal antibodies | View on Flintbox

    Antibodies raised against glutathione-S-transferase from Schistosoma japonicum which are rabbit polyclonal antibodies of the IgG isotype. Has been used for ELISA, westen blotting, immunoprecipitation, immunocytochemistry and immunofluorescence studies.

    Publications:

    Kalamajski S & Oldberg A Homologous Sequence in Lumican and Fibromodulin Leucine-rich Repeat 5-7 Competes for Collagen Binding. J Biol Chem 284:534-9 (2009). I-ELISA. PubMed: 19008226. www.jbc.org/

    Bansal PK et al. Sgt1 dimerization is negatively regulated by protein kinase CK2-mediated phosphorylation at S361. J Biol Chem : (2009). WB. PubMed: 19398558. www.jbc.org/

    Kathiresan T et al. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics 8:1972-87 (2009). PubMed: 19423573. www.mcponline.org/

    Scales TM et al. Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. J Cell Sci 122:2424-35 (2009). WB. PubMed: 19549690. jcs.biologists.org/

    Wood-Kaczmar A et al. An alternatively spliced form of glycogen synthase kinase-3beta is targeted to growing neurites and growth cones. Mol Cell Neurosci 42:184-94 (2009). WB; Rat. PubMed: 19607922.http://www.sciencedirect.com/science/journal/10447431

    Jones BW & Hinkle PM Subcellular trafficking of the TRH receptor: effect of phosphorylation. Mol Endocrinol 23:1466-78 (2009). PubMed: 19541745. mend.endojournals.org/

    Matheson LA et al. Correct targeting of plant ARF GTPases relies on distinct protein domains. Traffic 9:103-20 (2008). WB. PubMed: 17988226. www.traffic.dk/

    Bossard C et al. Dimeric PKD regulates membrane fission to form transport carriers at the TGN. J Cell Biol 179:1123-31 (2007). PubMed: 18086912. jcb.rupress.org/

  • Herpes Simplex Virus Type 1 (HSV-1) VP21/VP22a (scaffold) monoclonal antibody LP13

    Code: AB-0227

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP13 antibodies raised against Herpes Simplex Virus Type-1 VP21/VP22a scaffold protein, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein H monoclonal antibody BBH1

    Code: AB0223

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing BBH1 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein H, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot (no other anti-gH antibody detects protein by Western blot).

  • Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein B monoclonal antibody CB24

    Code: AB0226

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing CB24 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein B, which are mouse monoclonal antibodies of the IgG2b isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot.

  • KARPAS 929 Human Myeloma cell line

    Code: CL18

    | Cell lines | View on Flintbox

    Human Myeloma cell line useful in oncology research.

    Publications:

    Karpas et al., (2005) Studies of four new human myeloma cell lines. Leukemia & Lymphoma 46(1): 101-112.http://www.ingentaconnect.com/

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 417 Human Myeloma cell line

    Code: CL17

    | Cell lines | View on Flintbox

    Human Myeloma cell line useful in oncology research.

    Publications:

    Karpas et al., (2005) Studies of four new human myeloma cell lines. Leukemia & Lymphoma 46(1): 101-112.http://www.ingentaconnect.com/content/apl/glal?

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 25 Human Myeloma cell line

    Code: CL16

    | Cell lines | View on Flintbox

    Human Myeloma cell line useful in oncology research.

    Publications:

    Karpas et al. (2005). Studies of four new human myeloma cell lines. Leukemia & Lymphoma 46, 101-112.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 1272 Human Myeloma cell line

    Code: CL15

    | Cell lines | View on Flintbox

    Human Myeloma cell line useful in oncology research.

    Publications:

    Karpas et al., (2005) Studies of four new human myeloma cell lines. Leukemia & Lymphoma 46(1): 101-112.http://www.ingentaconnect.com/content/apl/glal?

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 241 Human Monoblastic cell line

    Code: CL20

    | Cell lines | View on Flintbox

    Human Monoblastic cell line useful in oncology research.

    Publications:

    Immunopharmacology 1982, 5(2) 123-7; Brit J Haematology 1980, 44, 415-424

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 620 Human Plasma Cell Leukaemia cell line

    Code: CL14

    | Cell lines | View on Flintbox

    Human Plasma Cell Leukemia cell line useful in oncology research.

    Publications:

    Nacheva et al. (1990). Br J Haematol 74, 70-76.

    Drexler and Matsuo (2000). Leuk Res 24, 681-703.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 45 Human T-cell Leukaemia cell line

    Code: CL13

    | Cell lines | View on Flintbox

    Human T-cell Leukemia cell line useful in oncology research.

    Publications:

    Smith et al. (1973). Lancet 301, 74-77.

    Borkhardt et al. (1997) Oncogene 14, 195-202.

    Drexler et al. (2004). Leukemia 18, 227-232.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 353 Human B-cell Leukaemia cell line

    Code: CL21

    | Cell lines | View on Flintbox

    KARPAS 353 Human B-cell Leukaemia cell line useful in oncology research.

    Publications:

    Nacheva et al. (1993) Blood 82, 231-240.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 231 Human B-cell Leukaemia cell line

    Code: CL9

    | Cell lines | View on Flintbox

    KARPAS 231 Human B-cell Leukaemia cell line useful in oncology research.

    Publications:

    Nacheva et al. (1993). Blood 82, 231-240.

    Galieque Zouitina et al. (1996). Leukemia 10, 579-587.

    Willis et al. (1997). Blood 90, 2456-2464.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 1718 Human Splenic Lymphoma cell line

    Code: CL22

    | Cell lines | View on Flintbox

    KARPAS 1718 Human Splenic Lymphoma cell line useful in oncology research.

    Publications:

    Cancer Research 2004 64 9 3087; http://cancerres.aacrjournals.org/

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 299 Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line

    Code: CL10

    | Cell lines | View on Flintbox

    KARPAS 299 Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line useful in oncology research.

    Publications:

    Ficsher et al. (1988). Blood 72, 234-240.

    Wolke et al. (2006). Int J Mol Med 17, 275-278.

    Drexler et al. (2004). Leukemia 18, 1569-1571.

    Mason et al. (1990). Br J Haematol 74, 161-168.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 384 Human T-cell non-Hodgkin's Lymphoma cell line

    Code: CL11

    | Cell lines | View on Flintbox

    KARPAS 384 Human T-cell non-Hodgkin's Lymphoma cell line useful in oncology research.

    Publications:

    Dyer et al. (1993) A new human T-cell lymphoma cell line (KARPAS 384) of the T-cell receptor gamma/delta lineage with translocation t(7:14) (p13;q11.2), Leukemia 7 1047-1053

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 422 Human B-cell Non-Hodgkin's Lymphoma cell line

    Code: CL12

    | Cell lines | View on Flintbox

    KARPAS 422 Human B-cell Non-Hodgkin's Lymphoma cell line useful in oncology research.

    Publications:

    Dyer et al. (1990). Blood 75, 709-714.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • KARPAS 1106P Human B-cell Non-Hodgkin's Lymphoma cell line

    Code: CL8

    | Cell lines | View on Flintbox

    KARPAS 1106P Human B-cell Non-Hodgkin's Lymphoma cell line useful in oncology research.

    A human B-cell lymphoma established from a pleural effusion of a 23-year-old woman with mediastinal lymphoblastic B-cell non-Hodgkin′s lymphoma.

    Publications:

    Nacheva et al. (1994). Blood 84, 3422-3428.

    Tamura et al. (1998). Int J Cancer 78, 100-105.

    Cambridge Enterprise Limited is the exclusive licensor for the Karpas cell line portfolio. We have partnered with ECACC (The European Collection of Cell Cultures; a Public Health England Culture Collection) to manage distribution of the majority of these cells on our behalf. Enquires to either organisation are welcomed.

  • W-12 cell line

    Code: CL2

    | Cell lines | View on Flintbox

    Human keratinocyte cell line useful in research related to human papillomavirus type 16 (HPV16) and cervical carcinoma.

    This reagent is available to license for internal research use only.

  • Sheep immunoglobulin G monoclonal antibody SIg 375

    Code: AB58

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing SIg 375 antibodies raised against sheep immunoglobulin G which are mouse monoclonal antibodies of the IgG isotype. Has been used in western blots, affinity chromatorgraphy and ELISA.

    Publications:

    Biochem J 1984, 219, 647-654

    J Neurochem 1987, 48, 622-630

    Nature 1987, 327, 232-234

    J Neurochem 1990, 55, 31-38

    J Neurochem 1993, 60, 219-227

  • Human Insulin-like growth factor-1 receptor monoclonal antibody IGF 1R 1-2

    Code: AB36

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 1-2 antibodies raised against the extreme C-terminus of the human insulin-like growth factor receptor beta subunit which are mouse monoclonal antibodies of the IgG2a isotype. Cross reacts with rat and mouse IGF-1R. Has been used in affinity chromatography, western blots and immunoprecipitation.

    Publications:

    Biochem J (1993) 290, 419

  • Human insulin receptor monoclonal antibody IR 18-34

    Code: AB3

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-34 antibodies raised against the extracellular domain of the human insulin receptor which are mouse monoclonal antibodies of the IgG2a isotype. Has been shown to cross react very weakly with bovine, porcine and ovine but not rabbit, rat or mouse insulin receptors. It has been shown to cause up to 50% inhibition of insulin binding and has been used in immunohistochemistry, immunoprecipitation and blocking assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Mouse Thy-1 monoclonal antibody YTS 154.7.7.10

    Code: AB10

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing YTS 154.7.7.10 antibodies raised against mouse Thy-1 which are rat monoclonal antibodies of the IgG2b isotype. Has been used in T+NK cell depletion in vivo and flow cytometry studies.

    Publications:

    Journal of Immunology Vol 134 5th May 1985 pages 3056-3061

    Cobbold S. & H. Waldmann (1986) Transplantation 41, 634

  • 4E3 - Antibody to human VEGF-A

    Code: AB84

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing 4E3 antibodies raised against human VEGF-A which are mouse monoclonal antibodies of the IgG1 isotype. Has been used for in vitro and in vivo neutralisation assays.

    Publications:

    Stephen Charnock-Jones et al. Novel antiangiogenic agents for use in contraception. 2005. Contraception 71: 263-271.www.contraceptionjournal.org/

    S. Charnock-Jones et al. Immunoneutralisation of vascular endothelial growth factor inhibits pregnancy establishment in the rhesus monkey (Macaca mulatta). 2007. Reproduction (2007) 133(6):1199. www.reproduction-online.org/

  • E7 protein of human papilloma virus 16 monoclonal antibody CamVir-3

    Code: AB56

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing CamVir-3 antibodies raised against E7 protein of Human Papilloma Virus 16 which are mouse monoclonal antiibodies of the IgG1 isotype. Has been used in western blots and immunohistochemistry.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Greenfield et al. 1991, PNAS 88, 11217-11221. www.pnas.org/

    McLean et al. 1993 Journal of General Virology, 74, 239-245. vir.sgmjournals.org/

  • Soybean clathrin heavy-chain monoclonal antibody 4A8

    Code: AB50

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing 4A8 antibodies raised against soybean clathrin heavy chain which are mouse monoclonal antibodies of the IgG1 isotype. Useful for the study of clathrin distribution in a variety of plant cell systems including Arabidopsis and tobacco. Has been used for western blotting, immunofluorescence and immunoelectron microscopy.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Blackbourn, HD and Jackson, AP. Plant clathrin heavy chain: sequence analysis and restricted localisation in growing pollen tubes. J. Cell Sci. 109, 777-787 (1996). jcs.biologists.org/

    Lam, BCH., Sage, TL., Bianchi, F and Blumwald, E. Role of SH3 domain-containing proteins in clathrin-mediated vesicle trafficking in Arabiopsis. The Plant Cell. 13, 2499-2512 (2001). www.plantcell.org/

    Lam, BCH., Sage, TL., Bianchi, F and Blumwald, E. Regulation of ADL6 activity by its associated molecular network. The Plant Journal 31, 565-576 (2002). onlinelibrary.wiley.com/journal/

  • Human muscle creatine kinase monoclonal antibody 15CH2 (anti-human CK-MM)

    Code: AB18

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing 15CH2 antibodies raised against human muscle creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157-1162, 1984. www.clinchem.org/

    Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310-315, 1985

    Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236-1241, 1986. www.ajconline.org/

    Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects--incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476-481, 1986. www.clinchem.org/

  • Human brain creatine kinase monoclonal antibody 2AC5 (anti human CK-BB)

    Code: AB17

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing 2AC5 antibodies raised against human brain creatine kinase which are mouse monoclonal antibodies of the IgG1 isotype. Useful for diagnosing tissue injury, especially myocardial infarction and potentially useful as a marker for head injuries, some neurological disorders and certain malignancies. Can also be used to generate a specific and sensitive assay for brain creatine kinase.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Jackson, A. P., Siddle, K., and Thompson, R. J. Two-site monoclonal antibody assays for human heart- and brain-type creatine kinase. Clin Chem, 30: 1157-1162, 1984. www.clinchem.org/

    Jackson, A. P. and Thompson, R. J. The stability of creatine kinase isoenzymes studied with two-site monoclonal antibody assays. Ann Clin Biochem, 22 ( Pt 3): 310-315, 1985.

    Rossi, R., Ekroth, R., Lincoln, C., Jackson, A. P., Thompson, R. J., Scallan, M., Tsang, V., Jackson, A., and Thompson, R. Detection of cerebral injury after total circulatory arrest and profound hypothermia by estimation of specific creatine kinase isoenzyme levels using monoclonal antibody techniques. Am J Cardiol, 58: 1236-1241, 1986. www.ajconline.org

    Thompson, R. J., Jackson, A. P., and Langlois, N. Circulating antibodies to mouse monoclonal immunoglobulins in normal subjects--incidence, species specificity, and effects on a two-site assay for creatine kinase-MB isoenzyme. Clin Chem, 32: 476-481, 1986. www.clinchem.org/

  • Human Z-alpha1 antitrypsin monoclonal antibody (anti-Z-AT clone 2C1)

    Code: AB-0200

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing 2C1 antibodies which detect the abnormal polymeric conformation of human alpha1 antitrysin but not the native monomeric protein. Potentially useful for detecting individuals with antitrypsin deficiency that can lead to cirrhosis of the liver or cancer. Has been used for ELISA & Western Blotting.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) VP16 (tegument) monoclonal antibody LP1 (anti-HSV VP16)

    Code: AB107

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP1 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 tegument protein VP16, which are mouse monoclonal antibodies of the IgG1 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot. VP16 is a transactivator present in the HSV virion. The linear epitope lies within the N-terminal 24 residues. This is the only known monoclonal antibody to VP16.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Major Capsid antigen (VP5) monoclonal antibody LP12 (anti-HSV VP5)

    Code: AB104

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP12 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 Major Capsid antigen (VP5), which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in Western blots.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein G monoclonal antibody AP1 (anti-HSV gG)

    Code: AB100

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing AP1 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein G monoclonal antibody LP10 (anti-HSV gG)

    Code: AB94

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP10 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein G, which are mouse monoclonal antibodies of the IgG3 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein E monoclonal antibody AP10 (anti-HSV gE)

    Code: AB101

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing AP10 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein E, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Herpes Simplex Virus Type 1 and 2 (HSV-1 and -2) Glycoprotein D monoclonal antibody LP2 (anti-HSV gD)

    Code: AB88

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP2 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG2a isotype. Strongly neutralises virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in Western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Minson AC, Hodgman TC, Digard P, Hancock DC, Bell SE, Buckmaster EA.
    An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
    J Gen Virol. 1986 67:1001-13.
    http://vir.sgmjournals.org/

    Buckmaster EA, Cranage MP, McLean CS, Coombs RR, Minson A. The use of monoclonal antibodies to differentiate isolates of herpes simplex types 1 and 2 by neutralisation and reverse passive haemagglutination tests.
    J Med Virol. 1984 13:193-202.
    http://eu.wiley.com/WileyCDA/WileyTitle/productCd-JMV.html

  • Herpes Simplex Virus Type 2 (HSV-2) Glycoprotein C monoclonal antibody LP6 (anti-HSV gC)

    Code: AB102

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP6 antibodies raised against Herpes Simplex Virus Type-2 envelope protein Glycoprotein C, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Mouse CD45 monoclonal antibody YW 62.3.20

    Code: AB62

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing YW 62.3.20 antibodies raised against mouse CD45 which are rat monoclonal antibodies of the IgG2b isotype.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Mouse CD5 (lyt-1) monoclonal antibody YTS 121.5.2

    Code: AB12

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing YTS 121.5.2 antibodies raised against mouse CD5 (lyt-1) which are rat monoclonal antibodies of the IgG2b isotype. Has been used in in vivo T cell depleting and flow cytometry studies.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Human beta 2-microglobulin monoclonal antibody YTH 470.5

    Code: AB81

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing YTH470.5 antibodies raised against human beta 2-microglobulin which are rat monoclonal antibodies of the IgG2b isotype.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Eur J Immunol (1988) 18:1507-1514

  • ISG 64 Antigen (invarient surface glycoprotein)

    Code: OM4

    | Other | View on Flintbox

    Recombinant protein produced in E. coli corresponding to the extracellular domain of ISG64. Soluble to >5 mg/ml in the absence of detergents. Potentially useful in the development of a serodiagnostic for human or animal infection with Trypanosoma brucei.

  • Recombinant mature form of human activin A growth factor produced in E. coli

    Code: OM1

    | Other | View on Flintbox

    It is a disulphide linked dimer with mass of 25 kDa and homogenious by SDS polyacrylamide gel electrophoresis. Useful in the regulation of cullular proliferation and differentiation and promotion of nueronal survival.

    Publications:

    Adrian E Harrington, Samantha A Morris-Triggs, Brandon T Ruotolo, Carol V Robinson, Shin-ichi Ohnuma and Marko Hyvonen. Structural basis for the inhibition of activin signalling by follistatin. EMBO J. 2006 25(5):1035-45.www.nature.com/emboj/index.html

  • E7 protein of Human Papilloma Virus 16 monoclonal antibody CamVir-4

    Code: AB57

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing CamVir-4 antibodies raised against E7 protein of Human Papilloma Virus 16 which are mouse monoclonal antiibodies. Has been used in western blots and immunohistochemistry.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Greenfield et al. 1991, PNAS 88, 11217-11221. www.pnas.org/

    McLean et al. 1993, Journal of General Virology, 74, 239-245. vir.sgmjournals.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 16-13

    Code: AB37

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 16-13 antibodies raised against the human insulin-like growth factor receptor (epitope 62-184) which are mouse monoclonal antibodies of the IgG2b isotype. Has been shown to enhance IGF binding and has been used for immunoprecipitation.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 17-69

    Code: AB38

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 17-69 antibodies raised against the human insulin-like growth factor receptor (epitope 440-586) which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause 35-60% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 24-55

    Code: AB33

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 24-55 antibodies raised against the human insulin-like growth factor receptor (epitope 440-586) which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause 35-60% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 24-57

    Code: AB34

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 24-57 antibodies raised against the human insulin-like growth factor receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause >80% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 24-60

    Code: AB35

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 24-60 antibodies raised against the human insulin-like growth factor receptor (epitope 184-283) which are mouse monoclonal antibodies of the IgG2a isotype. Has been shown to cause >80% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin-like growth factor-1 receptor monoclonal antibody IGF-1R 26-3

    Code: AB39

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 26-3 antibodies raised against the human insulin-like growth factor receptor (epitope 283-440 or 586-908) which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to enhance IGF binding and has been used for immunoprecipitation and immunohistochemistry.

    Publications:

    J Biol Chem (1992) 267, 12955. www.jbc.org/

    J Biol Chem (1993) 268, 1087. www.jbc.org/

  • Human insulin receptor monoclonal antibody IR 18-146

    Code: AB32

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-146 antibodies raised against the extracellular domain of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react very weakly with bovine, porcine and ovine but not rabbit, rat or mouse insulin receptors. It causes enhancement of insulin binding and has been used in immunoprecipitation assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 18-40

    Code: AB31

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-40 antibodies raised against the extracellular domain of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react weakly with bovine, porcine, ovine and rabbit, but not rat or mouse insulin receptors. It has been shown to cause up to 50% inhibition of insulin binding and has been used in immunoprecipitation and blocking assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 25-49

    Code: AB30

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 25-49 antibodies raised against the alpha subunit of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react weakly with rabbit but not bovine, porcine, ovine, rat or mouse insulin receptors. It has been shown to cause >80% inhibition of insulin binding and has been used in immunoprecipitation and blocking assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 47-9

    Code: AB2

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 47-9 antibodies raised against the alpha subunit of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react with bovine, porcine, ovine (and weakly with rabbit), but not with rat or mouse insulin receptors. It has been shown to cause >80% inhibition of insulin binding and has been used in blocking assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 83-12

    Code: AB41

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 83-12 antibodies raised against the extracellular domain of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react with bovine, porcine, ovine and rabbit, but not with rat or mouse insulin receptors. It has been shown to enhance insulin binding and has been used in immunoprecipitation assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 83-22

    Code: AB42

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 83-22 antibodies raised against the alpha subunit of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react very weakly with bovine, porcine and ovine, but not rabbit, rat or mouse insulin receptors. It has been shown to cause >80% inhibition of insulin binding and has been used in immunoprecipitation and blocking assays.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. http://www.jbc.org/

    J Biol Chem (1990) 265, 13248. http://www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR CT-3

    Code: AB5

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR CT-3 antibodies raised against the C-terminal portion of the human insulin receptor beta subunit which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cross react with rat and mouse insulin receptors and has been used in western blots and immunoprecipitation assays. This reagent is available to license for internal research only.

    Publications:

    Biochem J (1986) 235:199

    Biochem J (1987) 242:123

    EMBO J (1987) 6:4003

    Biochem J (1990) 268:615

    J Biol Chem (1990) 265:9970. http://www.jbc.org/

    J Biol Chem (1990) 265:13248 http://www.jbc.org/

    PNAS (1991) 88:9858. http://www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 18-42

    Code: AB76

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-42 antibodies raised against the beta subunit N-terminal region of the human insulin receptor which are mouse monoclonal antibodies of the IgG2b isotype. Has been used in immunoprecipitation assays.

    Publications:

    Biochem J (1986) 235:199

    Biochem J (1987) 242:123

    EMBO J (1987) 6:4003

    Biochem J (1990) 268:615

    J Biol Chem (1990) 265:9970 http://www.jbc.org/

    J Biol Chem (1990) 265:13248 http://www.jbc.org/

    PNAS (1991) 88:9858. http://www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 18-43

    Code: AB77

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-43 antibodies raised against the beta subunit N-terminal region of the human insulin receptor which are mouse monoclonal antibodies of the IgG2a isotype. Has been used in immunoprecipitation assays.

    Publications:

    Biochem J (1986) 235:199

    Biochem J (1987) 242:123

    EMBO J (1987) 6:4003

    Biochem J (1990) 268:615

    J Biol Chem (1990) 265:9970. http://www.jbc.org/

    J Biol Chem (1990) 265:13248. http://www.jbc.org/

    PNAS (1991) 88:9858. http://www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 18-45

    Code: AB78

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-45 antibodies raised against the beta subunit N-terminal region of the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been used in immunoprecipitation assays.

    Publications:

    Biochem J (1986) 235:199

    Biochem J (1987) 242:123

    EMBO J (1987) 6:4003

    Biochem J (1990) 268:615

    J Biol Chem (1990) 265:9970. http://www.jbc.org/

    J Biol Chem (1990) 265:13248. http://www.jbc.org/

    PNAS (1991) 88:9858. http://www.pnas.org/

  • Sheep immunoglobulin G monoclonal antibody SIg 482

    Code: AB59

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing SIg 482 antibodies raised against sheep immunoglobulin G which are mouse monoclonal antibodies of the IgG isotype. Has been used in western blots, affinity chromatorgraphy and ELISA.

    Publications:

    Biochem J 1984, 219, 647-654

    J Neurochem 1987, 48, 622-630

    Nature 1987, 327, 232-234

    J Neurochem 1990, 55, 31-38

    J Neurochem 1993, 60, 219-227

  • Sheep immunoglobulin G monoclonal antibody SIg 743

    Code: AB60

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing SIg 743 antibodies raised against sheep immunoglobulin G which are mouse monoclonal antibodies of the IgG isotype. Has been used in western blots, affinity chromatorgraphy and ELISA.

    Publications:

    Biochem J 1984, 219, 647-654

    J Neurochem 1987, 48, 622-630

    Nature 1987, 327, 232-234

    J Neurochem 1990, 55, 31-38

    J Neurochem 1993, 60, 219-227

  • Mouse Thy-1 monoclonal antibody YBM 29.2.1

    Code: AB11

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing YBM 29.2.1antibodies raised against mouse Thy-1 which are rat monoclonal antibodies of the IgG2c isotype. Has been used in flow cytometry and in vitro T cell depletion studies.

    Publications:

    Journal of Immunology Vol 134 5th May 1985 pages 3056-3061

    Cobbold S. & H. Waldmann (1986) Transplantation 41, 634

  • Herpes Simplex Virus Glycoprotein D monoclonal antibody AP12 (anti-HSV gD)

    Code: AB90

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing AP12 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG1 isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in Western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Minson AC, Hodgman TC, Digard P, Hancock DC, Bell SE, Buckmaster EA.
    An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
    J Gen Virol. 1986 67:1001-13.
    http://vir.sgmjournals.org/

  • Herpes Simplex Virus Glycoprotein D monoclonal antibody LP14 (anti-HSV gD)

    Code: AB89

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP14 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG2a isotype. Can be used to neutralise virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Can be used to detect protein by Western blot. The linear epitope has been defined for this antibody.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Minson AC, Hodgman TC, Digard P, Hancock DC, Bell SE, Buckmaster EA. An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
    J Gen Virol. 1986 67:1001-13.
    http://vir.sgmjournals.org/

  • Herpes Simplex Virus Glycoprotein D monoclonal antibody AP7 (anti-HSV gD)

    Code: AB92

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing AP7 antibodies raised against Herpes Simplex Virus Type-1 and Type-2 envelope protein Glycoprotein D, which are mouse monoclonal antibodies of the IgG2a isotype. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in Western blots. The antibody epitope is well characterised by binding competition assays and by sequencing of antibody escape mutants.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Minson AC, Hodgman TC, Digard P, Hancock DC, Bell SE, Buckmaster EA. An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization.
    J Gen Virol. 1986 67:1001-13.
    http://vir.sgmjournals.org/

  • Herpes Simplex Virus Glycoprotein H monoclonal antibody LP11 (anti-HSV gH)

    Code: AB103

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing LP11 antibodies raised against Herpes Simplex Virus Type-1 envelope protein Glycoprotein H, which are mouse monoclonal antibodies of the IgG2a isotype. Strongly neutralises virus in the absence of Complement. Works well for immunofluorescence (IF) on fixed specimens and for immunoprecipitiation (IP) on triton solubilised samples. Does not work in Western blots. Produces antibodies with unique anti-gH neutralising properties.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Buckmaster EA, Cranage MP, McLean CS, Coombs RR, Minson A. The use of monoclonal antibodies to differentiate isolates of herpes simplex types 1 and 2 by neutralisation and reverse passive haemagglutination tests.
    J Med Virol. 1984 13:193-202.
    http://eu.wiley.com/WileyCDA/WileyTitle/productCd-JMV.html

  • Human insulin-like growth factor 1 receptor monoclonal antibody IGF-1R 24-31

    Code: AB6

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IGF-1R 24-31 antibodies raised against human insulin-like growth factor 1 receptor (283-440 or 586-908 epitope) which are mouse monoclonal antibodies of the IgG1 isotype. Has been used in immunoprecipitation and blocking studies and causes

    This reagent is available to license for internal research only.

    Publications:

    J Biol Chem (1992) 267: 12955. www.jbc.org/

    J Biol Chem (1993) 268: 1087. www.jbc.org/

  • Human insulin receptor monoclonal antibody IR CT-1

    Code: AB4

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR CT-1 antibodies raised against the C-terminal portion of the human insulin receptor beta subunit which are mouse monoclonal antibodies of the IgG1 isotype. Has been used in western blots and immunoprecipitation assays.

    This reagent is available to license for internal research only.

    Publications:

    Biochem J (1992) 288, 195

  • Human insulin receptor monoclonal antibody IR 83-14

    Code: AB7

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 83-14 antibodies raised against the alpha subunit of the human insulin receptor which are mouse monoclonal antibodies of the IgG2a isotype. Has been shown to cause >80% inhibition of insulin binding and has been used in immunoprecipiatation and blocking assays.

    This reagent is available to license for internal research only.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 83-7

    Code: AB29

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 83-7 antibodies raised against the human insulin receptor which are mouse monoclonal antibodies of the IgG1 isotype. Has been shown to cause enhancement of insulin binding and has been used in immunoprecipitation assays.

    This reagent is available to license for internal research use only.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human insulin receptor monoclonal antibody IR 18-44

    Code: AB28

    | Monoclonal hybridomas | View on Flintbox

    Hybridoma cell line producing IR 18-44 antibodies raised against the beta subunit of the human insulin receptor which are mouse monoclonal antibodies of the IgG2b isotype. Has has been shown to cause up to 50% inhibition of insulin binding and has been used in western blots, immunoprecipitation and blocking assays.

    This reagent is available to license for internal research only.

    Publications:

    Biochem J (1986) 235, 199

    Biochem J (1987) 242, 123

    EMBO J (1987) 6, 4003

    Biochem J (1990) 268, 615

    J Biol Chem (1990) 265, 9970. www.jbc.org/

    J Biol Chem (1990) 265, 13248. www.jbc.org/

    PNAS (1991) 88, 9858. www.pnas.org/

  • Human CD18 monoclonal antibody YFC 118.12

    Code: AB79

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing YFC118.12 antibodies raised against human CD18 which are rat monoclonal antibodies of the IgG2b isotype

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

  • Human Leukocyte Antigen DR (HLA-DR) monoclonal antibody YAML 555.6

    Code: AB75

    | Monoclonal hybridomas | View on Flintbox | Download licence

    Hybridoma cell line producing YAML 555.6 antibodies raised against human leukocyte antigen DR (HLA-DR) which are rat monoclonal antibodies of the IgG2a isotype.

    A non-exclusive licence with standard terms and conditions is available for this reagent.

    To obtain a licence:
    1. Download the licence document via the link and enter your company name and full registered address on page 1 of licence agreement.
    2. Print two copies of the licence agreement and have both signed by an authorised official of your organisation.
    3. Provide your contact details (name, job title, email, telephone and fax number) for subsequent invoicing and mail both original signed licence agreements to:

    Research Reagents
    Cambridge Enterprise Limited
    University of Cambridge
    Hauser Forum
    3 Charles Babbage Road
    Cambridge CB3 0GT

    Publications:

    Journal of Immunology Vol 134 5th May 1985 pages 3056-3061